This study investigated the antiviral potential of fractions and eupomatenoid-5 obtained from Piper regnelli (Miq.) C. DC., Piperaceae, leaves against bovine herpesvirus-1 (BHV-1) and poliovirus. VERO cell monolayers in 96-well cell culture plates were infected with BHV-1 or poliovirus and incubated in the presence and absence of samples for 48 h. The cells were then fixed and stained with sulforhodamine B, and the virus-induced cytopathic effect was measured in a 96-well plate reader at 530 nm. Cytotoxicity was assessed by incubating the cell monolayers with samples for 72 h. The hexane, chloroform, chloroform/ethyl acetate (19:1), and chloroform/ethyl acetate (9:1) fractions showed activity against BHV-1. The chloroform, chloroform/ethyl acetate (19:1), chloroform/ethyl acetate (9:1), chloroform/ethyl acetate (1:1), and ethyl acetate fractions were active against poliovirus. The chloroform/ethyl acetate (9:1) fraction presented the best selectivity index for both viruses. The present study reports the antiviral activity of the extract and fractions of P. regnelli leaves.

Pfaffia glomerata (Spreng.) Pedersen, Amaranthaceae, is widely distributed in Brazil. Roots are considered as the world's greatest supplier and β-ecdysone is the most important compound extracted from roots of Pfaffia glomerata. So, the aim this study was analyze the presence of β-ecdysone in the inflorescences and stems and compared with the content from roots of Pfaffia glomerata and determine the best extractive method of β-ecdysone this plant. The crude extracts were obtained by Soxhlet method, reflux, maceration, percolation and turbolyse. Compound extracts were quantified by High Performance Liquid Chromatography (HPLC). The analysis were carried out a Phenomenex Column C18, 5 µm, 250x4,6mm, maintened at 30 ºC, gradient system using as mobile phase a mixture of methanol and water, flow rate 1,0 mL and detection at 245 nm. Results showed Soxhlet method with ethanol:water (90:10 v/v) presented the higher concentration of β-ecdysone in P. glomerata and inflorescences showed higher amount of this active substance (3,06%), compared with stems (2,37%) and roots (1,63%), showing that the inflorescences and plant stems may also be used as a rich source of β-ecdysone.

Um novo triterpeno, 3beta,22ksi,23ksi-triidróxi-22ksi-acetóxi-24-metil-lanosta-8,24(28)-dieno-31-al, denominado pisosteral, e quatro triterpenos lanostanos conhecidos, 3beta,22ksi,23ksi-triidróxi-22ksi-acetóxi-24-metil-lanosta-8,24(28)-dieno (pisosterol), 3alfa,22ksi,23ksi-triidróxi-22ksi-acetóxi-24-metil-lanosta -8,24(28)-dieno (3-epi-pisosterol), 3beta,22ksi,23ksi-triidróxi-23ksi-acetóxi-24-metil-lanosta-8,24(28)-dieno e 3beta,22ksi,23ksi-triidróxi-22ksi-acetóxi-24-etil-lanosta-8,24(28)-dieno, foram isolados do corpo de frutificação do fungo Pisolithus tinctorius, coletado em uma plantação de Eucalyptus. De uma linhagem de P. tinctorius isolada de Pinus taeda, foram isolados quatro triterpenos conhecidos derivados do lanostano, 3beta,22ksi-diidróxi-24-metil-lanosta-8,24(28)-dieno, 3beta,22ksi-diidróxi-24-etil-lanosta-8,24(28)-dieno, 3beta-hidróxi-lanosta-8,24-dieno (lanosterol) and 3beta-hidróxi-lanosta-7(8),9,24-trieno (agnosterol). Essas substâncias foram identificadas a partir de métodos químicos e físicos, principalmente espectrometria de massas e ressonância magnética nuclear 1D e 2D.

A new triterpene, 3beta,22xi,23xi-trihydroxy-24-methyllanosta-8,24(28)-diene-31-al 22-acetate, named pisosteral, and four known lanostane triterpenes derivatives, 3beta,22xi,23xi-trihydroxy-24-methyllanosta-8,24(28)-diene 22-acetate (pisosterol), 3alpha,22xi,23xi-trihydroxy-24-methyllanosta-8,24(28)-diene 22-acetate (3-epi-pisosterol), 3beta,22xi,23xi-trihydroxy-24-methyllanosta-8,24(28)-diene 23-acetate and 3beta,22xi,23xi-trihydroxy-24-ethyllanosta-8,24(28)-diene 22-acetate, were isolated from fruiting bodies of Pisolithus tinctorius, collected in an Eucalyptus plantation. From cultivation of a strain of P. tinctorius isolated from Pinus taeda, four known triterpenes lanostane derivatives, 3beta,22xi-dihydroxy-24-methyllanosta-8,24(28)-diene, 3beta,22xi-dihydroxy-24-ethyllanosta-8,24(28)-diene, 3beta-hydroxylanosta-8,24-diene (lanosterol) and 3beta-hydroxylanosta-7(8),9,24-triene (agnosterol), were isolated. These compounds were identified on the basis of chemical and physical methods, mainly mass spectrometry and 1D and 2D nuclear magnetic resonance.